Analysis Note

ControlPOSITIVE CONTROL:

Human tonsil

Application

Anti-Nuclei & Chromosomes, histone H1 protein, clone 1415-1 is a high quality Mouse Monoclonal Antibody for the detection of Nuclei & Chromosomes & has been validated in ELISA, WB, ICC, IHC(P).

Immunogen

Epitope: histone H1 protein

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

100 µL Hybridoma Supernatant, concentrated by ammonium sulfate. Buffer: PBS with 0.1% sodium azide.

Format: Purified

Specificity

Histone specific; stains nuclei and chromosomes of cells of all species including animals and plants. Reacts with preferentially with Histone H1 by ELISA and immunoblotting. MAB1276 produces a speckled nuclear staining pattern in all tissues studies.

Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse. Antibody reacts with rat cells {Bendeck, 2001}.